[RASMB] FW: diagnostic help - followup

[Peter Edward Prevelige Jr]

Thanks All -

It's reassuring to know that so many of my colleagues are in the lab and are their usually helpful selves during this holiday week.  

Thanks for all the help. Lake was closest. The counterbalance was properly aligned but apparently we have two and one is "broken". The good one and the "broken" one got swapped out by accident. 

Changing the counterbalance and recalibrating the delay solved the problem. 

Peter



Peter E. Prevelige Jr.
Professor
Dept. of Microbiology, BBRB 416/6
Univ. of Alabama @ Birmingham
845 19th St. South
Birmingham AL. 35294-2170
Phone 205 975-5327
FAX 205 975-5479
prevelig at uab.edu
http://www.microbio.uab.edu/faculty/prevelige/prevelige-p.htm


-----Original Message-----
From: Lake N. Paul, PhD [mailto:lpaul at purdue.edu] 
Sent: Tuesday, December 28, 2010 10:26 AM
To: Peter Edward Prevelige Jr
Cc: RASMB at rasmb.bbri.org
Subject: Re: [RASMB] diagnostic help

Merry Christmas and Happy Holidays!!!
Peter, Check the counter balance and see if it is aligned properly. 
Lake
-- 
Lake N. Paul, PhD
Biophysics Research Specialist
Discovery Park - Bindley Biosciences Center
Purdue University
1203 West State Street
West Lafayette, Indiana, 47905

BioAnalytical Lab Website:
http://www.purdue.edu/discoverypark/bioanalytical/

--------------------------------------------------------------------------------
"The great tragedy of Science - the slaying of a beautiful hypothesis by an ugly
fact." -- Thomas H. Huxley
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Quoting Peter Edward Prevelige Jr <prevelig at uab.edu>:

> Hi All -
> 
> I may just be having post or pre holiday haze but I could use some help. I am
> trying to run a sed V on a sample for someone. 3, 2 sector cells. Protein OD
> at 280 is ~0.8 as reported and checked by me, wavelengtn is set for 280,
> scans at 3K don't make sense. Too high, too noisy, no meniscus, etc.
> Instrument was working fine last week. I've attached screen captures of the
> scan and the actual data file from cell 1.
> 
> Any suggestions on what to check to sort this out?
> 
> Thanks
> 
> Peter Prevelige
> 
> Peter
>