[RASMB] dissociation of multiprotein complex in ultracentrifuge

[Mark Agacan]

Hello,
 
Very often when I run a velocity experiment at 45 K rpm with a multiprotein complex, I see peaks with MW values corresponding to the complex, and also to many of the individual components of the complex.  I have read the RASMB archives on the issue of pressure-induced dissociation of sedimenting ribosomes.  Do other people find this is happening for multiprotein complexes, perhaps those that are very weakly associated?  
 
Best Regards,
 
Mark
 
 
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Dr Mark Agacan, Scientific Officer for the Division of Biological Chemistry and Drug Discovery,
Wellcome Trust Biocentre, College of Life Sciences, University of Dundee, Dundee, DD1 5EH 
Tel: +44 1382 386095    Fax: +44 1382 345764    Mobile: 07525 451 117
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