[RASMB] Protocols Question

[Susumu UCHIYAMA]

 Hi

We usually use teflon plug gasket we made and hardly (almost never) experience leak problem.
We also have teflon gasket for aluminum centerpiece. This has different shape (only slightly small,
we re-designed this gasket not to get into double sector region when the top ring is screw up) from
Beckman type but fit well.

Best wishes,
Susumu

Susumu Uchiyama, Ph.D
Osaka University, Japan



(2010/09/02 2:47), Walter Stafford wrote:
> Hi AUCers,
>
> I have used neoprene gaskets since the early 1970's and have never had a leak attributable to the
> gaskets. I bought a 1 yrd x 1 yrd (smallest I could purchase) sheet of neoprene (0.0165 inches/
> 0.420 mm thick) and it has worked very well all these years. The neoprene is soft and flexible
> enough that it fills the scratches that inevitably appear around the filling holes - but it is
> tough enough that it doesn't tear or end up going down the holes (silicone rubber does).
>
> I punch the gaskets as I need them with a Sears leather/belt punch.
>
> Walter
>
> Tom Laue wrote:
>> Hi-
>> We use either teflon or neoprene gaskets sold by Spin Analytical www.spinanalytical.com, their
>> hex-head screws and a torque screwdriver (disclaimer, I am half-owner of Spin, but draw no
>> salary). Both teflon and neoprene are softer, so they seal better, especially with older
>> centerpieces that have scratches around the holes. The hex-head screws are much, much easier to
>> use than slot-headed screws, and the torque screwdriver ensures that you get a good seal without
>> deforming the housing.
>> Best wishes,
>> Tom
>>
>> On 8/31/2010 5:52 PM, Brian wrote:
>>> I am using the standard protocols listed on analyticalultracentrifugation.com for running AUC
>>> experiments. When I try to let me samples sit under vacuum to thermally equilibrate for an hour
>>> before running my scans some of my cells always leak past the red seals in the centerpiece fill
>>> ports. I don't see any mention of using any other types of cells or centerpieces. I am using 2
>>> channel epon centerpieces with 2 fill holes per channel from Beckman. I was wondering how
>>> everybody keeps their cells from leaking during this time.
>>>
>>> Thanks,
>>> Brian
>>>
>>
>