[RASMB] Weak association?

[Peter Schuck]

Hi Andrew,
this reminds me on Arthur's paper
http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=17483161
carbohydrates or not, the ideas on the sedimentation analysis should apply 
anyway.
Peter

At 09:22 AM 2/2/2009, Leech, AP wrote:
>Hello all,
>
>I have done some SV runs at various concentrations 0.3-10 mg/ml on
>a protein (~14 ka) and the sedimentation coefficient appears to
>remain constant (about 1.58). I had expected it to drop slightly at
>higher concentrations, and so I suspect a weak self association.
>Buffer is NaCl/Tris.
>
>Is it reasonable to try and estimate an association coefficient
>from this sort of data, and what is the best way to do it? (Even
>a lower limit would be acceptable, as the intent is to show that
>dimerisation is not significant).
>
>I'm working my way through Gilbert & Gilbert at the moment (Meth.
>Enzymol vol 27, p273) but is there perhaps something more in the
>"global analysis" line?
>
>All comments appreciated,
>
>Andrew
>
>--
>Dr Andrew Leech                  * Laboratory Manager
>Technology Facility              * Molecular Interactions Laboratory
>Department of Biology (Area 15)  * Tel  : +44 (0)1904 328723
>University of York               * Fax  : +44 (0)1904 328804
>PO Box 373, York YO10 5YW      * Email : apl3 at york.ac.uk
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