[RASMB] AUC Analysis for a protein without tryptophan

[Dmitry Veprintsev]

Hi Rikita,
in addition to the suggestion to use 230 nm, one could
1) inroduce a Trp if it is recombinant
2) label it with fluorescein and use 492 nm absorbance.
3) introduce Flash-tag as it may be more gentle then 2) (again, if it is
recombinant)
options 2 and 3 make it amendable for fluorescence detection system
regards, Dmitry

Ritika Sethi wrote:
> Hi
>  
> I am new to this technique.
> My protein precipitates in most of the buffers and one can't go beyond
> a certain (very low) concentration. I was hoping to screen this
> protein against different buffers and see micro-aggregates on the AUC
> and then try different additives to remove the microaggregates.
> However, I believe its impossible to do so if the protein is without
> tryptophan.
>  
> Any suggestions please?
>  
> Thanks
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-- 
Dr. Dmitry Veprintsev
MRC Laboratory of Molecular Biology
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