[RASMB] Query concerning membrane protein sedimentation equilibrium experiment

[Arthur Rowe]

Hi John (and everyone)

That sounds good to me. Of course you could have more than one protein
monomer contained in each micelle - but that should be immediately obvious
from the M value.

Kind regards

Arthur

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Arthur J Rowe
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From: John_Doran at vrtx.com
Date: Mon, 2 Nov 2009 16:46:47 -0500
To: rasmb at rasmb.bbri.org
Subject: [RASMB] Query concerning membrane protein sedimentation equilibrium
experiment



We have measured the amount of detergent bound to our membrane protein and
hence were able to calculate a Vbar for the protein detergent complex. Is it
sufficient to just substitute this value for Vbar along with the density
value of the detergent buffer solution into the ideal species equation of
heteroanalysis to get an accurate molecular weight value of the
protein-detergent complex, or is it more involved than that? Any thoughts
concerning this would be appreciated. Thank you.

John Doran 
Vertex Pharmaceuticals
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