[RASMB] Query concerning membrane protein sedimentation equilibrium experiment

[John_Doran at vrtx.com]

We have measured the amount of detergent bound to our membrane protein and 
hence were able to calculate a Vbar for the protein detergent complex. Is 
it sufficient to just substitute this value for Vbar along with the 
density value of the detergent buffer solution into the ideal species 
equation of heteroanalysis to get an accurate molecular weight value of 
the protein-detergent complex, or is it more involved than that? Any 
thoughts concerning this would be appreciated. Thank you.

John Doran
Vertex Pharmaceuticals
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://list.rasmb.org/pipermail/rasmb-rasmb.org/attachments/20091102/c8d05fd7/attachment.htm>