[RASMB] timing of auc runs

[John E Harlan]

Judith-

Are you recording absorbance scans?  If so, is the radial span you are 
scanning the same for the runs you are comparing?

John Harlan



Judith Kornblatt <judithk at alcor.concordia.ca> 
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09/25/2009 12:38 PM

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[RASMB] timing of auc runs






How consistent should the length of time for a run be? I've been doing 
the same basic run multiple times - same speed, same number of scans - 
and the total time for the run, as displayed on the panel of the AUC, 
varies considerably. For example, 200 scans, 32000 rpm, the times varied 
from 13:15 to 14:51. More recently, 200 scans at 37000, times varied 
from about 13 hours to 14 hours. Is this variation normal?
Judith Kornblatt
Chemistry and Biochemistry
Concordia University
Montreal Quebec

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