[RASMB] peculiar leakage problem!

sumit goswami ikant11 at gmail.com
Fri Jun 12 13:51:49 PDT 2009


Dear all
            we have been trying do some sed-velocity experiments for last
couple of weeks but every time we tried most of the cell assemblies leaked!!
Apparently it seemed like a simple problem of aging of center pieces but
then very recently I ran all the seven cell assemblies overnight at 50000rpm
with water in them and none leaked! After that I used the same set of
assembly for running my sample by sucking off the water from them  and 4 out
of 5 leaked even at 4000rpm. We have used different kinds of buffer so
far... PE (5mM Na-phosphate, 1mM EDTA), TE (10mM Tris, 1mM EDTA), Poly
buffer (MES,tris, acetate). All of our buffer had 0.01%PEG in them. We have
tried both PH 5 & 8.
   Can anyone suggest what's going on? We are ready to buy new centerpiece
but not sure if that's the problem. The result with water is confusing us!

Thank you very much!

Regards,
Sumit
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