[RASMB] changes of vbar during unfolding/refolding

[John Correia]

Sednterp has an option to calculate vbar in the presence of urea or guanidine.  This is probably based upon work primarily from Timasheff & coworkers.  As a start see reviews in Methods Enzymol vol 61:  chapter 3) Lee et al, chapter 4) Lee & Timasheff.  Also Prakash & Timasheff, Anal Biochem 117, 330 (1981).  Prakash et al ABB 210, 455 (1981).
 
As to intermediates there is some AUC data on this - maybe more with detergents but some data as a function of denaturant concentration - but you are now really in the realm of folders and need to ask a different audience about ensembles vs intermediates.
 
 

>>> On 3/28/2009 at 7:51 AM, in message <020501c9afa3$e216b9c0$a6442d40$@edu>, "Titus M. Franzmann" <tmfr at umich.edu> wrote:

Hello to everyone,
Is there information available about how vbar changes during chemical induced protein unfolding / refolding, or how to assess  the population of differently folded intermediates.
Thank you very much for your input. 
Best wishes
Titus
 
 
 
 
Titus M. Franzmann
University of Michigan
Molecular, Cellular, Developmental and Biology Department (MCDB)
830 North University
Ann Arbor, Mi, 48109, USA


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