[RASMB] more on dithionite and Hb

[Jack Kornblatt]

Hello Mitra
Chance, I think, once described dithionite as man's worst enemy. the 
concentration that you are using is far in excess of what is need to 
keep your solutions anaerobic. The reaction products of dithionite are 
too  numerous to list even if I could remember them. If you degas your 
solutions just before loading and then add dithionite to 1 mM this 
should give you the desired "low" oxygen.
Is it really necessary to keep dithionite as low as possible? I have 
little experience with Hb but if we use dithionite and cytochrome c 
oxidase at 11 mM there are sufficient biproducts generated that 
interpreting data is very difficult

best
jack kornblatt