[RASMB] peptide in DMSO

[Leech, AP]

Hi all,

Can I be the first to point out the viscosity of DMSO? I don't
have accurate figures but a quick web search implies >2 cp
(twice that for water) and variable with water content of the
mixture. The other thing is, how will you detect your peptide?
DMSO has quite a high UV absorbance so you might have difficulty
if your peptide doesn't have a decent extinction coefficient.
(Has anyone ever tried the interference optics with DMSO?)

Andrew

Tom Laue wrote:
> Hi Christine-
> A couple of things to keep in mind-
> The buoyancy of your peptide will be very small in 90% DMSO. See what 
> Steve, et al. used for the density at 90%.
> DMSO is compressible, so you may need to include compressibility in your 
> calculations (depending on rotor speed)
> Finally, DMSO is not entirely innocuous with epoxy. Your centerpieces 
> may get softened.
> Best wishes,
> Tom
> 
> Schar, Christine wrote:
>> Hi
>> I was hoping to get some advice about running a peptide (24mer, 2787 Da)
>> in 90% DMSO. I searched the archives and found Harding has used 90% DMSO
>> successfully for carbohydrates (not peptides) so I know my centerpieces
>> won’t melt at least.
>>
>> The group I’m working with used this peptide in NMR.  They didn’t get a
>> good signal in PBS so they switched to DMSO.  They’re seeing NOEs and 
>> they
>> are interested in knowing if their peptide aggregates.  Another
>> possibility is it forms micelles.  Is it feasible to try a run with this
>> peptide in DMSO?  Are there any special considerations?
>>
>> Thanks for any help.
>> Christine Schar
>>
>>
>> _______________________________________________
>> RASMB mailing list
>> RASMB at rasmb.bbri.org
>> http://rasmb.bbri.org/mailman/listinfo/rasmb
>>
>>   
> 

-- 
Dr Andrew Leech                   *  Laboratory Manager
Technology Facility               *  Molecular Interactions Laboratory
Department of Biology (Area 15)   *  Tel   : +44 (0)1904 328723
University of York                *  Fax   : +44 (0)1904 328804
PO Box 373,  York  YO10 5YW       *  Email : apl3 at york.ac.uk