[RASMB] green fluorescent protein dissociation constant (corrected)

[Arthur Rowe]

Dave -

Sorry, I must have mis-heard the name of the protein when you spoke to me on
the phone a few minutes ago.

The Kd for GFP has been estimated as 60 µM (Wiehler et al (2003) ChemBioChem
4 1164-1171) and more recently as 237 ± 36 µM with YFP at 100 ±19 µM by Zeng
et al - incl AJR -(2006) Biochemistry 45, 10482-10491. Pure statistical
errors are of course not the only factors to be taken into consideration
when comparing values. It may be that all these three values are not too
different from each other.

Arthur

more haste, less etc etc! Conclusion stands.

-- 
*************************
Arthur Rowe
Lab at Sutton Bonington
tel: +44 115 951 6156
fax: +44 115 951 6157
*************************

From: "Scott David" <Dj.Scott at nottingham.ac.uk>
Date: Fri, 25 Jul 2008 10:42:41 +0100
To: <rasmb at rasmb.bbri.org>
Subject: [RASMB] green fluorescent protein dissociation constant



Helllo to alll in RASMB-land

Does anyone know the green fluorescent protein dissociation constant
(preferably in PBS pH 7.0).

cheers! 
d. 

Dr. David J. Scott 
Lecturer in Physical Biochemistry
National Centre for Macromolecular Hydrodynamics
School of Biosciences
University of Nottingham
Sutton Bonington Campus
Sutton Bonington 
Leicestershire 
LE12 5RD 
United Kingdom 
dj.scott at nottingham.ac.uk
www.nottingham.ac.uk/ncmh <file://www.nottingham.ac.uk/ncmh>
+44 (0) 115 951 6221 Phone
+44 (0) 115 951 6142 Fax

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