[RASMB] DSC questions

[Rong-Rong Zhu]

Hello everyone,

 

I am very new in the DSC field and I have a few questions that I thought
someone here will be able to help me.

 

1) Has anyone run DSC with Manitol in the formulation buffer?  Are there
any potential problems associated with manitol?  

2) Has anyone run DSC of pegylated protein?  Do you expect high Tm and
more transition temperatures for pegylated protein vs non-pegylated
protein?

 

Many Thanks,

 

Rong-Rong

 

________________________________

From: rasmb-bounces at rasmb.bbri.org [mailto:rasmb-bounces at rasmb.bbri.org]
On Behalf Of John E Harlan
Sent: Wednesday, May 21, 2008 2:47 PM
To: John Burgner
Cc: rasmb at rasmb.bbri.org; rasmb-bounces at rasmb.bbri.org
Subject: RE: [RASMB] Broken XLA Mono

 


FYI- 

Last I knew Skip was at a company called Technical Alternatives, a third
party provider of service for a variety of lab equipment. 

John Harlan 



 

"John Burgner" <jburgner at purdue.edu> 
Sent by: rasmb-bounces at rasmb.bbri.org 

05/20/2008 10:03 PM 

        
        To:        "'John Gunther'" <jrg at mywdo.com>,
<rasmb at rasmb.bbri.org> 
        cc:        (bcc: John E Harlan/LAKE/PPRD/ABBOTT) 
        Subject:        RE: [RASMB] Broken XLA Mono




John, 
I had the same thing happen with our old XLA.  I was able gently shake
the monochromator while holding it in a horizontal  position and get the
filter out of the light path.  If you can locate Skip Perry, who was a
long-time Beckman service  man, he might be able to help out.   
John 
  
From: rasmb-bounces at rasmb.bbri.org [mailto:rasmb-bounces at rasmb.bbri.org]
On Behalf Of John Gunther
Sent: Tuesday, May 20, 2008 7:13 PM
To: rasmb at rasmb.bbri.org
Subject: [RASMB] Broken XLA Mono 
  
Dear AUC Techies 
   I picked up the Monochromator out of it's box and I heard a glass
like tinkling inside near the order filter. The order filter only moves
a small amount and won't go either way to it's normal stop. It's
apparent to me that something is broken. No one in the lab knows how
this happened. The last logged user got good results. It now will not
pass any energy. 
    The PI called Beckman and they offered to replace it for an exchange
price of $16,000.00. 
    Does anyone have experience repairing/disassembling and reassembling
one of these? Any hints, tips, or suggestions? 
  
John Gunther_______________________________________________
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