[RASMB] v-bar estimates/measurements for G-Quadruplexes

[Tom Laue]

Hi-
The partial specific volume is a system (not molecular) parameter. For 
poly-electrolytes, the partial specific volume depends on the solvent 
and, in particular, the counterions. We have done some work with a 
quad-stranded oligo in 100 mM KCl, 10 mM Tris, pH 8. The vbar we 
estimate from equilibrium data is 0.61+/-0.02 ml/g, which seems a bit 
high to me (I'd expect something closer to 0.55 ml/g). On the other 
hand, this is a G-C rich oligo.
Best wishes,
Tom

Cody Craig wrote:
> Hi All,
>
> Is there a good reference for estimating the partial specific volume 
> of deoxyguanosine/deoxyguanosine monophosphate in a quadruplex?  The 
> literature indicates that the observed partial specific volume of 
> nucleotides is context specific (single stranded is different than 
> double stranded), which makes me hesitant to use either of these 
> values for my calculations.
>
> Thanks in advance,
>
> Cody Craig
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-- 
Department of Biochemistry and Molecular Biology
University of New Hampshire
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Phone: 603-862-2459
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E-mail: Tom.Laue at unh.edu
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