[RASMB] peptide in DMSO

[Tom Laue]

Hi Christine-
A couple of things to keep in mind-
The buoyancy of your peptide will be very small in 90% DMSO. See what 
Steve, et al. used for the density at 90%.
DMSO is compressible, so you may need to include compressibility in your 
calculations (depending on rotor speed)
Finally, DMSO is not entirely innocuous with epoxy. Your centerpieces 
may get softened.
Best wishes,
Tom

Schar, Christine wrote:
> Hi
> I was hoping to get some advice about running a peptide (24mer, 2787 Da)
> in 90% DMSO. I searched the archives and found Harding has used 90% DMSO
> successfully for carbohydrates (not peptides) so I know my centerpieces
> won’t melt at least.
>
> The group I’m working with used this peptide in NMR.  They didn’t get a
> good signal in PBS so they switched to DMSO.  They’re seeing NOEs and they
> are interested in knowing if their peptide aggregates.  Another
> possibility is it forms micelles.  Is it feasible to try a run with this
> peptide in DMSO?  Are there any special considerations?
>
> Thanks for any help.
> Christine Schar
>
>
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