[RASMB] V-bar of DHPC

[James Lear]

Chi-Yaun

I've measured the Mw and vbar of DHPC micelles in 20mM acetate pH4 at  
25 deg C using AUC equilibrium interference scans over a range of  
different densities (using D2O) and globally fitting the data to a  
single species equilibrium model.  I got a vbar of 0.865 and micelle  
Mw of 9688.  Hope this helps

Regards,
Jim Lear

On Mar 30, 2007, at 9:57 AM, columbia wrote:

>> Dear all,
>>
>> Does anybody have any idea about the partial specific volume of  
>> dihexanoylphosphatidylcholine (DHPC)? And is there any difference  
>> in V-bar when DHPC become micelle? Our proteins will be  
>> dissociated in the presence of DHPC. The more DHPC, the more  
>> dissociation. I am thinking the possibilities of our protein  
>> binding to DHPC micelle. I appreciate if any comments.
>>
>>
>> Chi-Yuan Chou
>> Postdoctoral fellow,
>> Department of Biological Sciences, Columbia University, New York
>> 212-854-5221
>
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