[RASMB] new reductant profiles online

[David Hayes]

Hi,

I also wanted to report for the list that TCEP has not seemed as 
powerful as a reducing agent at DTT in some experiments here and we 
have gone back to DTT in some cases to ensure that disulfide 
crosslinks are broken.  We did not do a systematic study, but just 
quickly went back to DTT after trying TCEP because of the result we 
were getting in AUC and in some Malemide labelling reactions.

We use interference optics most of the time, so we don't worry as 
much about the absorbance.  We used the TCEP in the hope that 
Malemide labelling reactions could be performed more easily.

Dr. David B Hayes
Analytical Ultracentrifugation Research Laboratory
Boston Biomedical Research Institute
64 Grove St.
Watertown, MA  02472
617-658-7738