[RASMB] Sedimentation coefficient of human serum albumin monomer and good protein standard with well established s value

Tom Laue Tom.Laue at unh.edu
Mon Dec 17 12:58:47 PST 2007


Hi all-
Be careful with the Tanford data. The D20,w for RNAaseA is off (low as I 
recall) by 20%.
Serum albumin may not be a good standard due to its tendency to form 
dimers, and possible contamination by trace quantities of IgG.
I'll be interested in other's suggestions of possible standards... I've 
never met a protein that was well behaved, except under a limited range 
of conditions.
Best wishes,
Tom

John Burgner wrote:
> Yiming,
> On page 381 (Table 22-1) of Charlie Tanford's book "Physical Chemistry of
> Macromolecules" there is a list proteins with s20,w, D20,w, and vbar for a
> number of different macromolecules with s values from 1.6 to 170S.
>
> John Burgner
>
>
>
> -----Original Message-----
> From: rasmb-bounces at rasmb.bbri.org [mailto:rasmb-bounces at rasmb.bbri.org] On
> Behalf Of Yi-Ming_Li at hgsi.com
> Sent: Monday, December 17, 2007 1:50 PM
> To: rasmb at rasmb.bbri.org
> Subject: [RASMB] Sedimentation coefficient of human serum albumin monomer
> and good protein standard with well established s value
>
>
> Hi! Everyone
> We are looking for some "protein standard" to qualify our sedimentation
> velocity method. We think that HSA may be a good candidate for the
> standard. Does anyone know the sedimentation coefficient of human serum
> albumin monomer (corrected for buffer density and viscosity as well as
> vbar)? Some literatures said HSA has a sedimentation coefficient of 4.6 s,
> this is significantly higher than the value of the monomer sedimentation
> coefficient of HSA I got by analyzing the SV data of Sigma HSA using C(s)
> model. The C(s) profile of this Sigma HSA showed that the product contains
> about 80% monomer, 17% dimer and 3% trimer. So I guess the 4.6 s value
> reported in some literatures is the weight average apparent s value of the
> HSA monomer, dimer and trimer (or even some oligomers) in the sample
> instead of the apparent s value of HSA monomer. Therefore it will be very
> helpful for me if anyone can tell me the accurate s value of HSA monomer.
> Also the suggestions for better protein standard (better commercial
> available) with a well established s value will be very helpful.
>
> Thanks!
>
> Yiming
>
>
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