[RASMB] PIPES viscosity/density data

[Jack Kornblatt]

Hello all
If you would like to measure solution densities but do not have a few K 
to shell out for an Anton-Parr instrument, density/specific gravity 
bottles (picnometers) are still available. You calibrate them with 
mercury and the calibration lasts forever. They will give you at least 
five place accuracy provided your balances do not complain. We use them 
quite often but they are not meant to measure Vbar unless your proteins 
are dirt cheap.
best to all
jack kornblatt




John Correia wrote:

> Attached is a figure I produced years ago for the lab.  It is part of 
> a larger study we never followed up on because we measure the density 
> of every buffer we use in AUC.  So this was one 400 mM stock solution 
> at pH 6.9 used to make duplicate dilutions of each data point.  
> Measurements were on an Anton Paar DMA 5000.  Fits were done in 
> Origin.  I have some other temperature data as well but I have to put 
> that in an approachable format.
>  
> I encourage everyone to get a density meter and just measure each 
> buffer you use.
>  
>  
>  
> -------------------------------------------------------------------
> Dr. John J. "Jack" Correia
> Department of Biochemistry
> University of Mississippi Medical Center
> 2500 North State Street
> Jackson, MS  39216
> (601) 984-1522                                
> fax (601) 984-1501                            
> email address: jcorreia at biochem.umsmed.edu    
> homepage location: http://biochemistry.umc.edu/correia.html
> dept homepage location:    http://biochemistry.umc.edu/
> -------------------------------------------------------------------
>
>
>
>
> >>> On 11/29/2007 at 8:31 AM, in message 
> <4E1235AC63CA484CA744D086AF4B7E5C01E9BFA1 at lavexb01.am.boehringer.com>, 
> <psalois at lav.boehringer-ingelheim.com> wrote:
>
> Dear RASMB users
>
> I'm wondering if anybody has data about the viscosity/density of PIPES 
> buffer. I looked in the archives and, if I'm correct, this topic was 
> discussed a while ago (1998 ?)
>
> Any info would be appreciated
>
>
> Many thanks in advance
>
> Patrick Salois
> Research associate
> Boehringer Ingelheim Laval (Canada)
>
>
>
>
>
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