[RASMB] PIPES viscosity/density data

Thu Nov 29 07:53:14 PST 2007

We also follow Jack's approach, not only for the buffer, but also for the samples,
especially if you cannot readily determine vbars from peptide sequence or for
cases where DNA, synthetic polymers, detergents or carbohydrates are involved.

The latest version of UltraScan now includes a module for the calculations
of density series to aid those of us who have older Anton Paar densitometers that
still came without software.

-Borries

> Attached is a figure I produced years ago for the lab.  It is part of a lar=
> ger study we never followed up on because we measure the density of every b=
> uffer we use in AUC.  So this was one 400 mM stock solution at pH 6.9 used =
> to make duplicate dilutions of each data point.  Measurements were on an An=
> ton Paar DMA 5000.  Fits were done in Origin.  I have some other temperatur=
> e data as well but I have to put that in an approachable format.
>  =
> I encourage everyone to get a density meter and just measure each buffer yo=
> u use.
>  =
>  =
>  =
> -------------------------------------------------------------------
> Dr. John J. "Jack" Correia
> Department of Biochemistry
> University of Mississippi Medical Center
> 2500 North State Street
> Jackson, MS  39216
> (601) 984-1522                                 =
> fax (601) 984-1501                             =
> email address: jcorreia at biochem.umsmed.edu     =
> homepage location: http://biochemistry.umc.edu/correia.html =
> dept homepage location:    http://biochemistry.umc.edu/ =
> -------------------------------------------------------------------
> 
> 
> 
> 
> >>> On 11/29/2007 at 8:31 AM, in message <4E1235AC63CA484CA744D086AF4B7E5C0=
> 1E9BFA1 at lavexb01.am.boehringer.com>, <psalois at lav.boehringer-ingelheim.com>=
>  wrote:
> 
> Dear RASMB users =
> I'm wondering if anybody has data about the viscosity/density of PIPES buff=
> er. I looked in the archives and, if I'm correct, this topic was discussed =
> a while ago (1998 ?)
> Any info would be appreciated =
> 
> Many thanks in advance =
> Patrick Salois =
> Research associate =
> Boehringer Ingelheim Laval (Canada) =
> 
> 
> 
> 
> 
> 
> 
> Individuals who have received this information in error or are not authoriz=
> ed to receive it must promptly return or dispose of the information and not=
> ify the sender. Those individuals are hereby notified that they are strictl=
> y prohibited from reviewing, forwarding, printing, copying, distributing or=
>  using this information in any way.
> 
> 
> --=__Part5B7D5FE7.3__=
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> <DIV>Attached is a figure I produced years ago for the lab.  It is par=
> t of a larger study we never followed up on because we measure the density =
> of every buffer we use in AUC.  So this was one 400 mM stock solution =
> at pH 6.9 used to make duplicate dilutions of each data point.  Measur=
> ements were on an Anton Paar DMA 5000.  Fits were done in Origin.&nbsp=
> ; I have some other temperature data as well but I have to put that in an a=
> pproachable format.</DIV>
> <DIV> </DIV>
> <DIV>I encourage everyone to get a density meter and just measure each buff=
> er you use.</DIV>
> <DIV> </DIV>
> <DIV> </DIV>
> <DIV> </DIV>
> <DIV>-------------------------------------------------------------------<BR=
> >Dr. John J. "Jack" Correia<BR>Department of Biochemistry<BR>University of =
> Mississippi Medical Center<BR>2500 North State Street<BR>Jackson, MS  =
> 39216<BR>(601) 984-1522        &nbs=
> p;            &=
> nbsp;           <BR>fax (=
> 601) 984-1501          &n=
> bsp;           &nbsp=
> ;      <BR>email address: jcorreia at biochem.umsmed.=
> edu     <BR>homepage location: <A href=3D"http://bioche=
> mistry.umc.edu/correia.html">http://biochemistry.umc.edu/correia.html</A><B=
> R>dept homepage location:    <A href=3D"http://biochemistry.=
> umc.edu/">http://biochemistry.umc.edu/</A><BR>-----------------------------=
> --------------------------------------<BR><BR><BR><BR><BR>>>> On 1=
> 1/29/2007 at 8:31 AM, in message <4E1235AC63CA484CA744D086AF4B7E5C01E9BF=
> A1 at lavexb01.am.boehringer.com>, <psalois at lav.boehringer-ingelheim.com=
> > wrote:<BR></DIV>
> <DIV style=3D"PADDING-LEFT: 7px; MARGIN: 0px 0px 0px 15px; BORDER-LEFT: #05=
> 0505 1px solid; BACKGROUND-COLOR: #f3f3f3"><!-- Converted from text/rtf for=
> mat -->
> <P><SPAN lang=3Dfr-ca><FONT face=3DArial size=3D2>Dear RASMB users</FONT></=
> SPAN> </P>
> <P><SPAN lang=3Dfr-ca><FONT face=3DArial size=3D2>I'm wondering if anybody =
> has data about the viscosity/density of PIPES buffer. I looked in the archi=
> ves and, if I'm correct, this topic was discussed a while ago (1998 ?)</FON=
> T></SPAN></P>
> <P><SPAN lang=3Dfr-ca><FONT face=3DArial size=3D2>Any info would be appreci=
> ated</FONT></SPAN> </P><BR>
> <P><SPAN lang=3Dfr-ca><FONT face=3DArial size=3D2>Many thanks in advance</F=
> ONT></SPAN> </P>
> <P><SPAN lang=3Dfr-ca><FONT face=3DArial size=3D2>Patrick Salois</FONT></SP=
> AN> <BR><SPAN lang=3Dfr-ca><FONT face=3DArial size=3D2>Research associate</=
> FONT></SPAN> <BR><SPAN lang=3Dfr-ca><FONT face=3DArial size=3D2>Boehringer =
> Ingelheim Laval (Canada)</FONT></SPAN><SPAN lang=3Den-us></SPAN> </P></DIV>=
> <br>
> <br>
> <br>
> <br>
> Individuals who have received this information in error or are not authoriz=
> ed to receive it must promptly return or dispose of the information and not=
> ify the sender. Those individuals are hereby notified that they are strictl=
> y prohibited from reviewing, forwarding, printing, copying, distributing or=
>  using this information in any way.
> 
> </BODY></HTML>
> 
> 
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> ////////////////////////////////////////////////////////////////////////////
> ////////////////////////////////////////////////////////////////////////////
> ////////////////////////////////////////////////////////////////////////////
> ////////////////////////////////////////////////////////////////////////////
> ////////////////////////////////////////////////////////////////////////////
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> //8AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAoiwAAAAAAAAFAFMAdQBt
> AG0AYQByAHkASQBuAGYAbwByAG0AYQB0AGkAbwBuAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
> KAACAQIAAAAEAAAA/////wAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAB8AAAAA
> EAAAAAAAAAUARABvAGMAdQBtAGUAbgB0AFMAdQBtAG0AYQByAHkASQBuAGYAbwByAG0AYQB0AGkA
> bwBuAAAAAAAAAAAAAAA4AAIB////////////////AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
> AAAAAAAAAAAAJwAAAAAQAAAAAAAAAQBDAG8AbQBwAE8AYgBqAAAAAAAAAAAAAAAAAAAAAAAAAAAA
> AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAABIAAgEBAAAABgAAAP////8AAAAAAAAAAAAAAAAA
> AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAagAAAAAAAABPAGIAagBlAGMAdABQAG8AbwBsAAAA
> AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAFgABAP//////////////
> /wAAAAAAAAAAAAAAAAAAAAAAAAAAoBU9tpT4wgGgFT22lPjCAQAAAAAAAAAAAAAAAAAAAAAAAAAA
> AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
> AAAA////////////////AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
> AAAAAAAAAQAAAP7/////////////////////////////////////////////////////////////
> ////////////////////////////////////////////////////////////////////////////
> ////////////////////////////////////////////////////////////////////////////
> ////////////////////////////////////////////////////////////////////////////
> ////////////////////////////////////////////////////////////////////////////
> ////////////////////////////////////////////////////////////////////////////
> ////////////////////////////////////////////////////////////////////////////
> ////////////////////////////////////////////////////////////////////////////
> ////////////////////////////////////////////////////////////////////////////
> //////8BAP7/AwoAAP////8GCQIAAAAAAMAAAAAAAABGGAAAAE1pY3Jvc29mdCBXb3JkIERvY3Vt
> ZW50AAoAAABNU1dvcmREb2MAEAAAAFdvcmQuRG9jdW1lbnQuOAD0ObJxAAAAAAAAAAAAAAAAAAAA
> AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
> AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
> AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
> AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
> AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
> AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
> AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
> AAAAAA==
> 
> 
> --=__Part5B7D5FE7.2__=
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> MIME-Version: 1.0
> Content-Transfer-Encoding: 7bit
> Content-Disposition: inline
> 
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> --=__Part5B7D5FE7.2__=--
> 