[RASMB] nucleic acid vbar

[Arthur Rowe]

Yes, the vbar of nucleic acids depends heavily on the cation(s) present, and
can vary at least over the range 0.52 - 0.55 ml/g: Pearce, Rowe & Turnock,
(1975) J. Mol. Biol.  97  193-205. If the exact value matters to you, I
doubt that there is any way of avoiding doing an actual measurement, using a
modern DDM. 

Regards to all

Arthur


Bloomfield, Crothers & Tinoco show the salt dependence of vbar for Cs and
NaDNA (taken from Eisenberg (1976), so .55 is a traditional starting point,
not a fact.

>>> On 8/26/2007 at 2:56 PM, in message
<200708261956.l7QJuWtc008982 at biochem.uthscsa.edu>, Borries Demeler
<demeler at biochem.uthscsa.edu> wrote:
There seems to be a consensus to use a vbar of .55 ccm/g for DNA and RNA.
Does
anyone know if there is a function that predicts variations in vbar with
temperature
like there is for peptides? Or is .55 the best value to use - are there any
references on this?

Thanks, -borries
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