[RASMB] Question about the error in determination of kinetic parameters by SedAnal

[Joy Zhao]

Hello everyone,
I have a question about the error in determination of kinetic
parameters by SedAnal. When I tried to fit my SV data to obtain kr (or
kf) for a simple monomer-dimer system by using 2A-->A2 model, the
error for the kinetic parameter seems big. I am wondering whether this
is typical for the analyis by SedAnal.

(1).I was using F-statistics, Confidence Interval 68.27%. The rmsd of
the fitting is 4.6771032•10-3 .

(2). This global fitting was performed with four data sets.

(3). Results: kr=0.00238 S-1    min=0.001138 S-1        max=0.099988
S-1(CI=68.27%)

Thank you for your help!

Joy Zhao
Dept of Chem and Biochem
University of Maryland
College Park,MD 20742