[RASMB] XL/I absorbance problem

[Virgil Schirf]

Andrew,

There shouldn't be a problem switching out the monochromator to 
troubleshoot. I've done it before without incident. You will have to do 
a wavelength calibration if you do switch out the monochromators and 
that sometimes can take several scans to get the 230/567 nm peaks in the 
correct positions.

Attached is a picture of, what we termed, the dreaded comb similar to 
what you are seeing. This particular problem was caused by bad circuitry 
atop the heat sink after the instrument overheated causing major damage; 
however, other factors mentioned earlier can cause the same effect - 
most notably a bad or dirty slit drive.

Good luck,

Virgil



-- 

Virgil Schirf
Manager, CAUMA <http://cauma.uthscsa.edu> & CLS <http://cls.uthscsa.edu>
Department of Biochemistry, Rm 4.523U
The University of Texas Health Science Center
7703 Floyd Curl Dr.
San Antonio TX 78229-3900
Voice mail: 210 567-6590
Fax: 210 567-6595
Email: schirfv at biochem.uthscsa.edu <mailto:schirfv at biochem.uthscsa.edu>

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