[RASMB] densitometer

Jo Butler pjgb at mrc-lmb.cam.ac.uk
Tue Feb 20 02:12:59 PST 2007


Dear Eray,

I can certainly help with an answer to the question about density 
meters.  One very good one, measuring to precision of 10**-6 g/ml, is 
the density meter DMA60, with a DMA602 cell, (from Anton Paar, Graz, 
Austria).  The sample volume is slightly less than 1ml.  There may be 
other ones with similar precision, but I do not know of them.
One other thing which you do absolutely need with this is a 
thermostatting system, capable of giving a liquid flow through the 
jacket of the cell and accurate (particularly stable) to 0.01°C, in 
order to prevent fluctuations in density due to the temperature 
dependence of the solvent (usually water).

Yours,

Jo

Eray Dalgakiran wrote:
> Hi!
>  
> We have recently purchased a ProteomeLab XL-I analytical 
> ultracentrifuge. We are dealing with synthetic polymers, proteins, 
> biopolymers, and complexes of protein-polymer molecules.
>  
> So far, I understand that we have to know partial specific volume of 
> solute material to make calculations. We now want to buy a 
> densitometer in order to measure partial specific volumes. Can 
> anyone recommend me a good densitometer (company, model etc.)?
>  
> And is there anyone knows the v-bar value of PAA (Polyacrylic acid)?
>
> Many thanks...
>  
>  
>  
> Eray Dalgakiran
>  
> Department of Bioengineering
> Yildiz Technical University
>
> www.bioeng.yildiz.edu.tr <http://www.bioeng.yildiz.edu.tr>
> ------------------------------------------------------------------------
>
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>   

-- 
Dr P.J.G. Butler,
MRC Laboratory of Molecular Biology,
Hills Road, Cambridge, CB2 2QH, UK.
Tel. +44 (0)1223 402296




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