[Fwd: Re: [RASMB] pH stability]

[Dmitry Veprintsev]

-------- Original Message --------
Subject: Re: [RASMB] pH stability
Date: Thu, 25 Jan 2007 07:19:43 -0600 (CST)
From: Borries Demeler <demeler at biochem.uthscsa.edu>
To: dbv at mrc-lmb.cam.ac.uk (Dmitry Veprintsev)

To all:

For a quick reference, we have the chemical compatibility table
from Beckman for aluminum and epon charcoal filled centerpieces
reproduced on our website (among several other useful AUC resources):

http://www.cauma.uthscsa.edu/

(click on resources, you can find it in the left tab).
Aluminum is definitely not good with NaOH, but low concentrations
(5%) are listed OK with epon.

Another possibility may be the titanium centerpieces from Kristian
Schilling - perhaps Kristian can comment on their stability with
various NaOH concentrations.

-Borries

> 
> Dear Yasuaki,
> from my experience the copper screws welded to the aluminum cell case:
> Aluminium easily dissolves in alkali.
> Our workshop had to drill them out and re-tap the holes.
> Quartz windows could also be etched by strong NaON but that wold not be
> as fast.
> You may have better luck with 6-sector cells.
> regards, Dmitry
> 
> Yasuaki Hiromasa wrote:
> > Dear RASMB members,
> > I am working with peptide/protein at basic condition. I want to
> > characterize it at pH10-12 or 1-10 mM NaOH. Can I apply under these
> > condition to Analytical ultracentrifuge?  Will these base conditions
> > hurt the cell assembly?
> > We have aluminum and epon centerpieces. Windows are quartz and sapphire.
> > We want to do velocity and equilibrium experiments.
> > Has anybody experience at these conditions?
> > 
> > Sincerely,
> > Yasuaki HIromasa
> > Department od Biochemistry
> > Kansas State University
> > 
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> 
> -- 
> Dr. Dmitry Veprintsev
> MRC Centre for Protein Engineering
> Hills Road, Cambridge CB2 2QH, UK
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> 



-- 
Dr. Dmitry Veprintsev
MRC Centre for Protein Engineering
Hills Road, Cambridge CB2 2QH, UK