[RASMB] AUC with RNA

[Cole, James]

Dear Dror:
We have successfully used the RNAseZAP product from Ambion mentioned by
Virgil to clean centerpieces and windows to study RNAs and RNA-protein
complexes by AUC. In some cases, when you add proteins they contain
trace RNase contamination that can hydrolyze RNA. In these cases it can
be useful to include  trace amounts of an RNase inhibitor protein. This
stuff is sold by Ambion, Epicentre or Promega. Another thing to think
about is checking your annealling conditions. Hairpins are very prone to
form dimers. Depending on what you are planning to do, you may also need
to experimentally measure the extinction and v-bar of your RNA. 
Good luck,

Jim Cole

-----Original Message-----
From: rasmb-bounces at rasmb.bbri.org [mailto:rasmb-bounces at rasmb.bbri.org]
On Behalf Of Dror Noy
Sent: Thursday, December 21, 2006 5:55 AM
To: rasmb at server1.bbri.org
Subject: [RASMB] AUC with RNA

Hi All,
A colleague has interested me in a project that may involve AUC
measurements of RNA. I have good experience with measuring proteins but
none with RNA. I'd greatly appreciate any information, through this
forum or directly to my email, about working with RNA in the analytical
ultracentrifuge particularly any special considerations that are
different from protein work.
Thanks,
Dror



=========================
Dror Noy, Ph.D.
Plant Sciences Dept.
Weizmann Institute of Science
Rehovot 76100
Israel

e-mail: dror.noy at weizmann.ac.il
Tel: (972)-8-934 4300
Fax: (972)-8-934 4184


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