[RASMB] Should we fit the SV data for bottom level for quantifying protein aggregate?

[Peter Schuck]

Yiming,
if the data range you are considering for analysis contains regions of 
back-diffusion for any component, then yes, otherwise no.
Peter


Yi-Ming_Li at hgsi.com wrote:
> Hi:
> Based on the results of analyzing many SV data for quantifying protein
> aggregates with Sefit Software, I observed that the change of bottom level
> setting influences the quantitation of aggregates. According to the Step by
> Step Tutorial in the Sefit website, we need to fit the data for friction
> ratio, meniscus level and time independent noise but not bottom level.
> Since manual setting of bottom level may result in some error in bottom
> level, I would like to know whether we should also fit the data for bottom
> level. Thanks!
>
> Yiming
>
>
> _______________________________________________
> RASMB mailing list
> RASMB at rasmb.bbri.org
> http://rasmb.bbri.org/mailman/listinfo/rasmb
>
>
>