[RASMB] sedimentation velocity in acetonitrile/water

Peter Schuck pschuck at helix.nih.gov
Wed Jun 14 05:28:21 PDT 2006


Hi Dror,
regarding the analysis of sedimentation velocity considering solvent 
compressibility, you will find information in
http://www.analyticalultracentrifugation.com/compressibility_of_water_and_organic_solvents.htm
and
Biophysical Chemistry  108:187-200  
(http://www.analyticalultracentrifugation.com/images/CompressibleSolvents.pdf)
Peter


Dror Noy wrote:
> Dear all,
> I'd be happy to get any information/tips/comments about running and 
> analyzing sedimentation velocity experiments of proteins in 
> acetonitrile/H2O/TFA mixtures. Is this feasible at all? How to deal 
> with compressibility issues? etc.
> Thanks,
> Dror
> =========================
> Dror Noy, Ph.D.
> Structural Biology Dept.
> Weizmann Institute of Science
> Rehovot 76100
> Israel
>
> e-mail: dror.noy at weizmann.ac.il
> Tel: (972)-8-934 2525
> Fax: (972)-8-934 4154
>
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