AW: [RASMB] Working with toluene

[Claus Urbanke]

Dear Thomas,
 
I did sedimentation equilibrium in Toluene and n-Hexane several years ago.
Judging from the data sheet I refrained from using epon centerpieces but
rather used Kel-F mantled Aluminum centerpieces and had no problem with
swelling or distortion. These centerpieces have been in the prodiction line
of Beckman with part # 331420 many years ago.
 
Hope this may help
 
Claus
 
 
 

Prof. Dr. Claus Urbanke

Medical School Hannover

Biophysical Chemistry OE 8830

D-30625 Hannover

Germany

 

 

 

  _____  

Von: rasmb-bounces at rasmb.bbri.org [mailto:rasmb-bounces at rasmb.bbri.org] Im
Auftrag von Thomas Adam Jowitt
Gesendet: Montag, 20. Februar 2006 17:25
An: rasmb at rasmb.bbri.org
Betreff: [RASMB] Working with toluene



Hello

 

I would like to know if anyone has any experience of working with toluene in
Epon centrepieces. I think there may be an issue with swelling of the
centrepiece in certain organic solvents which I assume could cause the cells
to break. As this is a velocity study the run length is quite short, also is
it possible to minimise problems by pre-swelling the centrepiece?

 

Any advice would be much appreciated

 

Thanks

 

Tom 

 

 

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