[RASMB] glycosylation

[Jacob Lebowitz]

Dear All,

The Lewis and Junghans and Shire, S.J. approaches are the same 
methodological analysis using the measurement of buoyant molar mass but the 
equations are identical but cast differently. I have used the Lewis, M. S., 
and R. P. Junghans approach several times for viral glycoproteins with very 
good results. In order to determine the molar mass of the glycoprotein you 
need to know the molar mass of the protein. Obviously no problem if your 
protein is a monomer but if you have an oligomer and want to determine 
stoichiometry you have to test different models. You probably can 
discriminate oligomeric states based on a reasonable value for the average 
glycosylation per N and O linked sites. This worked for me, however it is 
wise to get the molar mass of the glycoprotein monomer from mass spec to 
confirm the SE results.

Jack


At 01:38 PM 11/14/2005 -0800, Dyche Mullins wrote:
>Dear All,
>
>In digging through the RASMB archives I found the following references:
>
>Lewis, M. S., and R. P. Junghans. 2000. Ultracentrifugal analysis of 
>molecular mass of glycoproteins of unknown or ill defined carbohydrate 
>composition. Methods Enzymol 321:136-49.
>
>and
>
>Shire, S.J., 
><http://www.beckmancoulter.com/Literature/BioResearch/DS_837.pdf>http://www 
>.beckmancoulter.com/Literature/BioResearch/DS_837.pdf
>
>Which answered all my questions.
>
>Next time I'll check the archives first.
>
>xoxo
>Dyche Mullins
>UCSF
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