[RASMB] liposome

[Kirk C Aune]

Richard M Thomas wrote:

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> Dear Christine,
> Can't help much, but some years ago I did play with liposomes (LUVs) 
> although never with any success. I don't see why you shouldn't try 
> too, hopefully with better results - whether you get anywhere may 
> depend on composition, size and encapsulated material. There are some 
> things to bear in mind - the most prominent being vbar. vbars for 
> typical phospholipids are high which means that, certainly for SUVs, 
> you should observe flotation while, as the vesicles become larger you 
> may move close to neutral buoyancy - we observed both. All our 
> vesicles were 'empty'; entrapment of other components would also have 
> an effect depending on their composition and concentration.
> I have no idea what might happen if you move to the GUV size range and 
> would interested in hearing whether anybody has any views on this.
>
> Regards to all
> Richard

The following article might be relevant to what you are doing:

Physical Properties of the Dimyristoylphosphatidylcholine Vesicle and of 
Complexes Formed by Its Interaction with Apolipoprotein C-III.  
Biochemistry, (1977) 16, 2151.  Kirk C. Aune, Joseph G. Gallagher, 
Antonio M. Gotto, Jr. and Joel D. Morrisett

Kirk C. Aune