[RASMB] FC43 fluorocarbon silicon oil

[Tom Laue]

Hi Joris,
FC-43 is perfluoro-tertbutyl amine. It was available from 3-M. The 
material, as it is sent out, may contain some hydrocarbon contaminants. 
These can be removed by extracting the FC-43 with neat sulfuric acid, 
followed by rinsing with distilled-deionized water until there is no 
change in its pH.
Best wishes,
Tom

Beld, Joris wrote:

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>Dear all,
> 
>Maybe we are old fashioned but we still like to put a little bit (20-30 ul) of FC43 fluorocarbon silicon oil in the bottom of the sample sector (two sector centerpiece) during a sedimentation equilibrium run. Just to create a false bottom and prevent scatter problems at the edge (bottom) of the cell. Works fine and has been used a lot in the past. Lately we are almost running out of the oil, so I contacted Beckman and they told me the following:
> 
>"The fluorocarbon oil (306394) was used as part of the cell assembly and
>added to the sample side of the 6- channel centerpiece to "lift" the sample in
>the outer most sector above the cell bottom in order to collect more data in
>that area. This approach was abandoned when the cell holes were
>realigned shortly after the product launch near 1993 to better match the optical
>track of the instrument. I don't recall the exact date, but the oil has been
>discontinued for several years with no recommended replacement since it is no
>longer needed."
> 
>Does anyone know what the composition of this FC43 fluorocarbon silicon oil is (or does someone knows another source from which we can buy it)?  (I asked Beckman but so far they do not want to give us the composition)
> 
>Of course I can forget about the oil and just do runs without it but for reproducibility and comparison with previous experiments we would really like to have some.
> 
>Thanks in advance.
>Kind regards,
> 
>Joris Beld
>Swiss Federal Institute of Technology
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Department of Biochemistry and Molecular Biology
University of New Hampshire
Durham, NH 03824-3544
Phone: 603-862-2459
FAX:   603-862-0031
E-mail: Tom.Laue at unh.edu
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