[RASMB] AUC reproducibility

[pjgb at mrc-lmb.cam.ac.uk]

Dear Mircea,

We regularly repeat analyses of aggregating macromolecular samples, 
frequently from a different preparation, and find that the results are 
reproduceable.  (Indeed, we regard this as a requirement for publication of 
any analysis.)
If they are not, I want to know why not, as it typically means that some, 
previously unknown, factor is uncontrolled.

Yours,

Jo

--On Friday, April 1, 2005 8:01 pm +0200 "Rasa, M.V" <m.rasa at tue.nl> wrote:

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> Dear colleagues,
>
> I am investigating now the reproducibility of measurements
> of a sample prepared from the same supramolecular complex solution and
> salt solution,
> in ideal equilibrium runs and successive measurements (cells are
> refilled after cleaning).
> The whole procedure, including running parameters, is the same. Since I
> obtained contradictory results,
> I would like to ask you about your experience with reproducibility of
> measurements with XL-A.
> There are up to now no signs that something happened with our sample in
> time (from wavelength scan measurements).
> The chemical compatibility is also checked (well established already)
> and preparation avoided
> other polymers.
>
> Of course this is a general question, and I am very likely not aware of
> all factors which may affect the result,
> so I will come back with the necessary details according to your
> comments.
>
> Thank you very much.
>
> Sincerely yours,
>
> Mircea Rasa.
>
> ____________________________________________
>
> Mircea RASA
>
> Eindhoven University of Technology
> Faculty of Chemical Engineering and Chemistry
> Laboratory of Macromolecular Chemistry and Nanoscience
> PO Box 513, STO 3.39
> 5600 MB Eindhoven, The Netherlands
>
> Tel: +31 (0)40 247 2835
> Fax: +31 (0)40 247 4186
> E-mail: m.rasa at tue.nl
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P.J.G. Butler,
MRC Laboratory of Molecular Biology,
Hills Road, Cambridge, CB2 2QH, UK.
Tel. +44 (0)1223 402296