[RASMB] gravity sweep experiment

[Qin Zou]

Thanks Jack. That is very helpful. I'll try to play around. Are you going to
this year's biophysical society meeting or the Gibbs conference? Hope I
could talk to you then.
Chin
 
  _____  

From: John Correia [mailto:jcorreia at biochem.umsmed.edu] 
Sent: Saturday, January 29, 2005 1:35 PM
To: heat-capacity at indy.rr.com; rasmb at server1.bbri.org
Subject: Re: [RASMB] gravity sweep experiment
 
Chin
 
Walter is on vacation and may not be checking email from down under.  
 
To my knowledge WD-DCDT is the only program that can analyze multiple speed
velocity data globally.  Setting up to use it depends upon the range of s
values your sample has.  Check the Biophys Chem paper Stafford & Braswell
wrote & appreciate if you scan a narrow region of the cell, say 6.49 to
7.01, to catch the rapidly sedimenting species, you will need to go back
after the run and scan the meniscus region as a function of speed to correct
for rotor stretching.  To set up a WD run you also must use the sed equil
button in the Beckman software since that is the only place multiple speed
data collection can be programmed.  The analysis assumes all the data is in
continuously #'ed & in one directory.  WD-DCDT can also be found in the
SEDANAL package.
 
 
-------------------------------------------------------------------
 Dr. John J. "Jack" Correia
 Department of Biochemistry
 University of Mississippi Medical Center
 2500 North State Street
 Jackson, MS  39216
 (601) 984-1522                                 
 fax (601) 984-1501                             
 email address: jcorreia at biochem.umsmed.edu     
 homepage location: http://biochemistry.umc.edu/correia.html
 dept homepage location:    http://biochemistry.umc.edu/
-------------------------------------------------------------------
 
 

>>> "Qin Zou" <heat-capacity at indy.rr.com> 01/29/05 10:20AM >>>
Dear all,
Has some one tried to run the  gravity sweep experiment? How to set up the
experiment in XL-I? Can WINDCDT+ is the only one that can be used to analyze
the data? Thanks a bunch.
 
Chin
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