[RASMB] (no subject)

[Neil Errington]

Dear John

We have a Jasco 810 here that I have been using for the last 2 years. It doesn't
have all of the kit on that was in your spec but I know of a couple of other
labs which between them do have pretty much all of it.

None of us are unhappy with the kit as far as I'm aware. It is pretty reliable,
easy to use and the service (at least on our instrument) has been no problem at all.

I haven't used any of the other instruments you mentioned other than having seen
the Aviv spec running at a Workshop so I can't realy comment on those. But the
810 should do just what you want.

Best Regards

Neil
-- 
Dr. Neil Errington
Biomolecular Sciences Department
UMIST
PO Box 88, 
Manchester, 
M60 1QD, UK 
Tel: +44 (0)161 200 4191
Fax: +44 (0)161 236 0409 


Quoting John Burgner <jwb at bilbo.bio.purdue.edu>:

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> 
> Dear All,
> 
> Does anyone with recent experience with "state of the art" CD spectrometers
> have strong feelings on which  instruments would best fulfill the
> description given below. 
> Based on an intial investigation, it lookes like both  the Aviv 215 and the
> Jassco J-810 fit the bill. Both Olis and Applied Photophysics seem to be
> close 
> 
> 
> Based on a survey of proven and potential users, high-quality sample
> temperature control with automatic temperature ramping is an absolute
> requirement for all.  A rapid stopped flow system (less than or equal to 1
> ms dead time) is also essential for several users and will provide the fast
> kinetic circular dichroism capabilities that are currently absent from
> campus.  An automatic microtitrator will eliminate tedious and error-prone
> manipulations and provide for time-saving automated data collection.  This
> last capability was requested by several users, particularly for ligand
> binding studies and the determination of protein and nucleic acid stability
> as a function of denaturant concentrations.  Automatic titration will also
> require a device for the effective mixing of sample and titrant in the
> cuvette.
> 
> An additional optics and data processing package to allow the detection of
> fluorescence polarization and anisotropy in the same instrument would be a
> very valuable addition.  This polarization and anisotropy capability is not
> replicated anywhere else on campus.  Since these packages also allow
> collection of total fluorescence emission, this addition would enhance the
> current fluorescence stopped flow capabilities on campus.  The fluorescence
> package will allow simultaneous collection of circular dichroism and
> fluorescence signals on the same sample, providing immediate confirmatory
> data and reducing sample preparation requirements.  When combined with the
> proposed automatic titrator, this package would enable stability and ligand
> binding studies by complementary methods on samples in precisely the same
> state (e.g. same ligand and/or denaturant concentration).  
> 
> Thanks for your help
> John
> 
> John W. Burgner
> Department of Biological Sciences
> Purdue University
> 915 West State St
> West Lafayette, IN 47907-2054
> 765-494-4960
> 765-496-1189 (fax) 
> 
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