[RASMB] PSV of Phosphatidylethanolamine
Lustig
ariel.lustig at unibas.ch
Tue Jan 20 08:55:00 PST 2004
Dear Jo, dear colleagues ,
as far I understood you need the Vbar of Phospholipids.
To determine the Vbar of Phospholipids their density is very low , you
need a relative high concentration to get a good signal, and you have to
know the concentation to determine it in pycnometers -density-meters ! In
such a case I use the methode of:
Ching hsiens Huang+james P.Charlton
STUDIES ON PHOSPHATIDYCHOLINE VISICLES
Journ of Biol.Chemestry vol 246 ,April 25 1971, 2555-2560
The conc. knowledge is not important and conc. dependence is not needed.
I made it many times with schlieren opt. but with interfer. it will go too.
yours.... ariel
Lustig Ariel
Dept. of Biophysical Chemistry
Biozentrum der Universität Basel
Klingelbergstr. 70
CH 4056 Basel
Switzerland
,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
for other purposes :
Analytical Ultracentrifuge Lab.TEL....+61 2672187
Fax +61 2672189
E-mail ariel.lustig at unibas.ch
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