[RASMB] Pipette tips for filling centerpieces.

[John Philo]

David and all,
 
That was me, and I get those tips from E&K Scientific in Campbell,
California; 800-934-8114, www.eandkscientific.com. Like many good
discoveries, I found these by accident (I thought I had ordered the usual
tapered gel loading tips). 
 
E&K categorizes them as 'sequencing tips', shown under the 'specialty tips'
section of liquid handling supplies in their catalog and on their web site.
They are described as '200 microliter for Pipetman - 0.024 inch OD'. The
basic catalog number is 3597, and then there are different options for
whether you want them in bags, racks, or sterile in racks. There is also a
100 microliter 'RNase and DNase free' version as catalog #6697.
 
I think other companies probably carry equivalents, but all I can say is
that these do reach the bottom without enlarging the fill holes. I find that
with these it is also easier to fill the channel quite full---I routinely
load 440+ microliters of sample. 
 
Best regards,
 
John

-----Original Message-----
From: rasmb-admin at server1.bbri.org [mailto:rasmb-admin at server1.bbri.org] On
Behalf Of David Hayes
Sent: Thursday, July 08, 2004 4:07 PM
To: Rasmb
Subject: [RASMB] Pipette tips for filling centerpieces.


Hi all,
 
I was at the AUC Workshop and Symposium in Connecticut this year.  In a
moderately crowded room, someone said they used gel loading pipette tips
that were thin enough to reach the bottom of cells when loading.  (The gel
loading pipette tips I have tried only reach part way down the cell and
almost always spill sample.  Another lab I asked said they drilled out the
filling holes to make them wider)  
 
If someone can give me the name and supplier of these extra thin gel loading
pipette tips I would really appreciate it.
 
Thanks
 
David Hayes
 

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