[RASMB] fringe deviation

[lelj]

Hello!

I am trying to determine the association constant for a monomer-dimer equilibrium. I know the association occurs because I've seen it by velocity experiments.

I run equilibrium at 3 concentrations and 3 speeds, my conc. are high and therefore I used interference optics. I have a fringe displacement at equilibrium of up to 50 fringes. I fit my data with WinNonlin and at the best I can get a SQUARE ROOT OF VARIANCE=9.3317E-02.  Since I have such a big fringe displacement when I plot deviation vs. indipendent variable, in the worst case I have a deviation of -0.25 to 0.375 fringes, is it reasonable?

thanks 
barbara

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Barbara Lelj Garolla Di Bard
Dr. Mauk's Lab
Dept. of Biochemistry and Molecular Biology
University of British Columbia
2146 Health Sciences Mall
Vancouver, B.C. V6T 1Z3
Phone: (604) 822-2526