[RASMB] PSV of Phosphatidylethanolamine

Lustig ariel.lustig at unibas.ch
Tue Jan 20 08:55:00 PST 2004


Dear Jo, dear colleagues ,
as far  I understood  you need  the Vbar of Phospholipids.
To determine the Vbar of Phospholipids their  density  is very low , you 
need a relative  high concentration to get a good signal, and you  have to 
know the  concentation to determine it in  pycnometers -density-meters ! In 
such  a case  I use  the methode of:

Ching hsiens Huang+james P.Charlton
STUDIES  ON  PHOSPHATIDYCHOLINE  VISICLES
Journ of Biol.Chemestry vol 246 ,April 25 1971, 2555-2560

The conc. knowledge is not important  and conc. dependence is not needed.
I made it many times with  schlieren opt. but with interfer. it will go too.
yours.... ariel
Lustig Ariel
     Dept. of Biophysical Chemistry
   Biozentrum der Universität Basel
   Klingelbergstr. 70
   CH 4056 Basel
   Switzerland
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for other  purposes :

Analytical Ultracentrifuge Lab.TEL....+61 2672187
                                              Fax    +61 2672189
                                              E-mail ariel.lustig at unibas.ch
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