[RASMB] why use different loading concentration for sedimentation equilibrium

[Lin Fang]

Thanks all.

I have another question as I need to incorportate more concentration. 
and need to compare my wild type sample and mutant sample. so 
theoretically need to run them at the same time. Therefore, I 
consider use the 6 channel cell. To use this do I need to change the 
speed and loading volume? Thank you.


Lin


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> 
> Hi, 
> 
> I am investigating the oligermer status of my interest protein. And 
I
> noticed a lot use different loading concentration at one run and
> combine the data sets and fit them. I want to know what more
> information can more than one loading concentration can give me and
> use what program to fit this group of data.
> 
> 
> Thanks a lot
> 
> 
> Lin
> 
> 
> 
> 
> 
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