[RASMB] Help

[ospivey at bmb-fs1.biochem.okstate.edu]

Dear Guozhou,

    I suspect that someone does know this density.  However, if they 
don't, you will be able to assume that the density is only 
significantly different from that of water if the total solute 
concentration is "quite" high.  I and others could give you still 
better  estimates based on similar electrolytes if the density 
HEPES, is not known, but I think it will be known by others.  In 
fact, I think Borries Demeler's program has a lot of these data, but 
we would also need to know the exact composition and concentrations 
of ALL solutes in your HEPES buffer

Best wishes,
Olin



At 11:36 AM -0400 4/8/03, Guozhou Chen wrote:
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>Hi,
>I am using analytical ultracentrifugation to study my protein. I need
>calculate the solvent density of HEPES which is not listed in BECKMAN
>manual. Does anyone have the parameter for HEPES? Thanks a lot!
>
>Best regards,
>
>Guozhou Chen          mailto:gchen at bio.indiana.edu
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>Biology Department, Indiana University
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