Antwort: Re: [RASMB] sedimentation

Fri Oct 24 08:11:00 PDT 2003

Hi Irene,

the upper limit of particle diameters that can be addressed by AUC depends
- of course -
very much on the density your particles exhibit, the density and viscosity
of the solvent etc.
But from our experience as a rule of thumb 5 µm is a reasonable number for
latex particles
(With increasing diameter of the particles to be investigated slower speeds
have to be chosen,
but therefore - of course - the 1g earth's gravitational force acting
perpendicular to the gravitational
field caused by rotor spinning has to be taken into account more and more.
This is in practice the
reason for the existence of an upper limit in particle size analysis as
long as the Beckman type
of ultracentrifuge geometry is applied).

Regards
Lars

                      "Irene Hallyburton"                                                                                                   
                      <i.hallyburton at dundee         An:      rasmb at rasmb-email.bbri.org                                                     
                      .ac.uk>                       Kopie:   (Blindkopie: Lars Boerger/ZK/BASF-AG/BASF)                                     
                      Gesendet von:                 Thema:   Re: [RASMB] sedimentation                                                      
                      rasmb-admin at rasmb-ema                                                                                                 
                      il.bbri.org                                                                                                           

                      24.10.2003 14:09                                                                                                      

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Hi All

In the same vein as Jan's silica particles, a client has been using latex
microparticles in the development of Point of Care assays. He has
asked me about looking at them, coated and uncoated, in the AUC.
They will be quite large, and we did consider DLS, but our instruments
max is 50nm and they'll be bigger than that.

What is the upper size range for AUC?

Many Thanks

Irene

<0>-<0>-<0>-<0>-<0>-<0>-<0>-<0>-<0>-<0>-<0>
Irene Hallyburton
Post Genomics & Molecular Interactions Centre
Faculty of Life Sciences
University of Dundee
01382 348700

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