[RASMB] time to reach equilibrium

[Jo Butler]

Dear Patrick,

It is certainly a reasonable approximation to take an absence of further 
change in the distribution as indicating that an operational equilibrium 
has been reached.  There is, however, the caveat that the time interval 
between the overlaid scans is sufficient in comparison to the total time to 
equilibrium - running long column equilibrium runs I typically use 24hr 
intervals, as the runs take a matter of days to equilibrate (even with 
initial overspeeding).

Yours,

Jo

--On Wednesday, June 4, 2003 13:58 +0200 Patrick England 
<england at pasteur.fr> wrote:

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> Dear RASMB mailing list members,
>
> A general question regarding sedimentation/diffusion equilibria...
> What is, in your experience, the most effective way to determine and/or
> predict the time needed for the equilibrium to be reached at a given rotor
> speed?
> In practice, do you consider that the equilibrium is attained when no
> change can be observed in the concentration boundary for a certain length
> of time, or when the equilibrium is theoretically reached with a certain
> confidence interval?
>
> Many thanks for your replies!
>
> Sincerely yours,
> Patrick England
>
> -------------------------------------------------------------------------
> -- Patrick ENGLAND
>
> Responsable de la Plate-forme de Biophysique des Macromolécules et de
> leurs Interactions,
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P.J.G. Butler,
MRC Laboratory of Molecular Biology,
Hills Road, Cambridge, CB2 2QH, UK.
Tel. +44 (0)1223 402296