[RASMB] Fw: Trace interpretation ?

[Gordon Campbell]

----- Original Message -----
From: "Olwyn Byron" <o.byron at bio.gla.ac.uk>
To: "Gordon Campbell" <gordon at mblab.gla.ac.uk>
Sent: Friday, April 04, 2003 4:54 PM
Subject: Re: Trace interpretation ?


>    Dear RASMB'ers,   Could anyone assist in the interpretation of some
> scans that I  have obtained from a Sedimentation Velocity experiment ?
> This is the 2nd study that I have done on this particular  sample.   The
> 1st study showed, through SV & SE experiments, that  the sample was a
5kDa,
> monomer. The runs were performed @ 4degC in PBS buffer.  The partial
> specific volume of the sample was calculated to be 0.721, and the  buffer
> density was 1.007.   The second study was performed at the same
> temperature, but  the buffer in this case was 1:1 PBS/Trifluoroethanol,
for
> which I calculated the  density to be 1.22322.    300ul of sample was
> loaded into a double sectored cell and  sedimented @ 50k rpm.   Can anyone
> explain the interesting nature of the traces  ?   I've attached the traces
> as a word document,   Any help would be much appreciated,   thanks,
> Gordon Campbell,
> >IBLS, Division of Infection &   Immunity,
> >Joseph Black Building
> >University of Glasgow,
> >University  Avenue,
> >Glasgow G12 8QQ
> >Tel : 0141 339 8855 ext 0735
> >email : Gordon.Campbell at bio.gla.ac.uk
> >
> >Attachment converted: Macintosh HD:raw sv.doc (WDBN/MSWD) (000D2009)
>
>
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