[RASMB] V bar of haemoglobin

[Lustig]

Dear colleagues, dear Dave  Scott,...(Hi)
over years we  used  at the  Biozentrum  for  student courses  and 
other   studies 0.74 mL /gr, and got
Mw masses  of  64 Kda + /  - 1 at the  linear OD  410, 540 range in 
PBS  7.4  or similar buffers.
One have to take in consideration  that  at  extreme Low concentration  the 
molecule  dissociates  to 2 subunits, and  at high salt  0r extreme pH's 
the  dissociation starts  even at normal  concentrations. We have also to 
pay attention  that at high  salt  hydration  shifts  to  the range  of 
0.78 as other proteins  do.

Values can be  found  e.g. in a book or ask  the author  (you know him!) 
for a copy of the
  pages of interest)
Durchschlag,H. ,in Themodynamic data for  Bioch. +Biotechn.
(Hinz, H.H., ED.) Springer Verlag , Berlin -Heidelberg  New-York-Tokio , 
1986, pp 45-128)

In Press of BBA is  a  new  article using  0.74 :

Towards a resolution of the long-standing controversy regarding the 
molecular mass of extracellular erythrocruorin of the earthworm Lumbricus 
terrestris


Ezra Daniel*, Ariel Lustig, Melvyn M. David, Yossi Tsfadia

Department of Biochemistry
George S. Wise Faculty of Life Sciences
Tel-Aviv University, Tel-Aviv 699978, Israel
Department of Biophysical Chemistry, Biozentrum
University of Basel, CH-4056 Basel, Switzerland



       *Corresponding author:  Fax:    +972-3-6406834
                               Tel:    +972-3-6409441
                               E. mail: danielez at post.tau.ac.il


  Keywords: Lumbricus erythrocruorin; earthworm erythrocruorin;
            Lumbricus hemoglobin; earthworm hemoglobin;
            Molecular mass; central subunits.


  Lustig Ariel
Biozentrum der Universität Basel
Klingelbergstr. 70
CH 4056 Basel
Switzerland
Dept. of Biophysical Chemistry  /
Analytical Ultracentrifuge Lab.TEL....+61 2672187
                                Fax    +61 2672189
E-mail   Ariel.Lustig at unibas.ch

  Yours....ariel