[RASMB] Hb vbar...

[Arthur Rowe]

Hi Dave (and all RASMBers)

The partial specific volume of the haem (a.k.a. heme) group is 0.82 ml/g as
determined by ab initio calculation from volume increments (Mayer, Anderka,
Ludwig & Schubert; Proc Colloid Polym Sci (2002) 119: 77-83). You can
download this paper from:

www.biozentrum.uni-frankfurt.de/molgen/pdf/Mayer_PCPS_2002.pdf

They were looking at the cytochrome bc1 complex, but I doubt that the Hb
environment would make much difference. Get the total vbar for Hb by simple
weighting (see above reference).

Arthur

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Arthur J Rowe
Professor of Biomolecular Technology
NCMH Business Centre
University of Nottingham
School of Biosciences
Sutton Bonington
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Hello everyone in RASMB-land.

One question that was asked of me by an old colleague that I am unable
to answer:

What is the vbar of haemoglobin? Surely the haem groups make a
contribution and we cannot simply estimate it from the amino acid
sequence.

cheers

Dave Scott.

p.s. answers such as "go and measure it" are not feasible as he does
not have access to a density meter!


------------------------------
------------------------------
Dr. David J. Scott
Department of Biochemistry
School of Medical Sciences
University of Bristol
Bristol BS8 1TD
United Kingdom
Phone: +44 (0)117 954 6873
Fax:   +44 (0)117 928 8274
Email: david.scott at bristol.ac.uk
Web:   http://www.bch.bris.ac.uk/staff/halford/djswebpage.htm
...so why don't we drop food on them instead?


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