[RASMB] GST aggregation

[mmorris]

Dear John,

Try the following article which describes GST dimerisation (by
chromatography and the analytical) and the polymerisation of GST fused
to leucine zipper motifs.

Riley LG, Ralston GB, Weiss AS, "Multimer formation as a consequence of
separate homodimerization domains: the human c-Jun leucine zipper is a
transplantable dimerization module", Protein Eng 1996 Feb;9(2):223-30

Erratum in:Protein Eng 1996 Sep;9(9):831

Yours, Michael


John Rodgers wrote:

> I have received a sample for equilibrium analysis.  The protein of
> interest is bound to GST.  Does anyone know how strongly it might self
> associate, its Kd, or other interesting info.  My concern is that the
> GST might muddy the results at best. Thanks John Rodgers
-------------- next part --------------
A non-text attachment was scrubbed...
Name: michael.morris.vcf
Type: text/x-vcard
Size: 347 bytes
Desc: Card for mmorris
URL: <http://list.rasmb.org/pipermail/rasmb-rasmb.org/attachments/20020923/0cc4f307/attachment-0007.vcf>