[RASMB] Overspeeding to establish equilibrium

[John Correia]

The equations for time to equilibrium originally appeared in a 1958
vanHolde & Baldwin paper J Phy Chem 62: 734 (1958) - see also the
Yphantis ANYAS 88: 586 (1960) paper -  Every decrease of 2 in coulumn
height (3 mm to 1.5 mm) gains you a factor od 4 in time to equilibrium. 
 I bet short column in general will be faster than any overspeed
procedures??   Depends upon the information content you need, ie how
complicated is the association??  & if it really is unstable you still
have to collect data vs time & check to see how stable the curvature of
the data is.   


-------------------------------------------------------------------
 Dr. John J. "Jack" Correia
 Department of Biochemistry
 University of Mississippi Medical Center
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 email address: jcorreia at biochem.umsmed.edu     
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>>> Andrew Leech <A.Leech at uea.ac.uk> 06/25/02 01:03PM >>>
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Is there a procedure, or rule of thumb, to decide how fast to
run the AUC to minimise the time taken to reach equilibrium?

I am thinking about unstable samples and how to avoid the long
delays before reaching equilibrium.

Andrew

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Dr Andrew Leech                  *Tel +44 (0) 1603 593585
Scientific Officer               *Fax +44 (0) 1603 592250
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