[RASMB] low Mw peptide
Jacob Lebowitz
lebowitz at helix.nih.gov
Tue Apr 16 17:24:01 PDT 2002
Heather,
A belated response to your analysis of a low Mw peptide. You might consider
a sedimentation velocity experiment for the peptide using Peter Schucks
program Sedfit to determine Mw by fitting for s and D. Lamm equation
fitting worked very well for small peptides, see Schuck et al. 1998
Biophys. J. 466-474. The paper came before Peter developed sedfit. I
presume that you have Sedfit, if not go to
www.analyticalultracentrifugation.com.
Jacob Lebowitz, PhD
Molecular Interactions Resource
Division of Bioengineering and Physical Science, ORS
National Institutes of Health
Mail: Bldg. 13 Rm. 3N17
Office Bldg. 13 Rm. 3E49
13 South Drive
Bethesda, MD 20892 - 5766
Tel: (301) 435-1955
Fax: (301) 480-1242
email: lebowitz at helix.nih.gov
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